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PALLD ELISA Kit (Human) (OKCD00284)

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96 Wells
$525.00
In Stock
Application:
ELISA
Gene Symbol:
PALLD
Official Gene Full Name:
palladin, cytoskeletal associated protein
NCBI Gene Id:
23022
Alias Symbols:
CGI151, CGI-151, FLJ22190, FLJ38193, FLJ39139, FLJ61376, KIAA0992, MYN, Palladin, PNCA1, Sarcoma antigen NY-SAR-77, SIH002
Description of Target:
Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.3 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.10"Characterization of human palladin, a microfilament-associated protein."_x005F_x000D_ Mykkaenen O.-M., Groenholm M., Roenty M., Lalowski M., Salmikangas P., Suila H., Carpen O._x005F_x000D_ Mol. Biol. Cell 12:3060-3073(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH EZR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.Ref.11"Molecular analysis of the interaction between palladin and alpha-actinin."_x005F_x000D_ Roenty M., Taivainen A., Moza M., Otey C.A., Carpen O._x005F_x000D_ FEBS Lett. 566:30-34(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH ACTN.Ref.18"Palladin interacts with SH3 domains of SPIN90 and Src and is required for Src-induced cytoskeletal remodeling."_x005F_x000D_ Ronty M., Taivainen A., Heiska L., Otey C., Ehler E., Song W.K., Carpen O._x005F_x000D_ Exp. Cell Res. 313:2575-2585(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH AND SRC, SUBCELLULAR LOCATION, PHOSPHORYLATION.
Protein Name:
palladin
Swissprot Id:
Q8WX93
Protein Accession #:
NP_001159580
Nucleotide Accession #:
NM_001166108.1
Species Reactivity:
Human
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
< 0.051 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level PALLD were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level PALLD were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology PALLD ELISA Kit (Human) (OKCD00284) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PALLD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PALLD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PALLDcaptured in well.
Kit Component:
ComponentAmount
Anti-PALLD Microplate96 Wells (12 x 8 Well Strips)
PALLD Lyophilized Standard2 x 10 ng
100X Biotinylated PALLD Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PALLD.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PALLD.
Datasheets / Downloads:
Printable datasheet for OKCD00284
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Palladin (PALLD). No significant cross-reactivity or interference between Palladin (PALLD) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
1383
Protocol:
Tips Information:
 
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