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NSF ELISA Kit (Human) (OKCD00877)

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96 Wells
$545.00
In Stock
Application:
ELISA
Gene Symbol:
NSF
Official Gene Full Name:
N-ethylmaleimide sensitive factor, vesicle fusing ATPase
NCBI Gene Id:
4905
Alias Symbols:
NEM-sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, SKD2, Vesicle-fusing ATPase, Vesicular-fusion protein NSF
Description of Target:
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).By similarity
Protein Name:
vesicle-fusing ATPase
Swissprot Id:
P46459
Protein Accession #:
NP_006169
Nucleotide Accession #:
NM_006178.3
Species Reactivity:
Human
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
< 0.117 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level NSF were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level NSF were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology NSF ELISA Kit (Human) (OKCD00877) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NSF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NSF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NSFcaptured in well.
Kit Component:
ComponentAmount
Anti-NSF Microplate96 Wells (12 x 8 Well Strips)
NSF Lyophilized Standard2 x 20 ng
100X Biotinylated NSF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NSF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NSF.
Datasheets / Downloads:
Printable datasheet for OKCD00877
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF). No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
744
Protocol:
Tips Information:
 
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