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NSF ELISA Kit (Human) (OKCA01589)

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96 Wells
$600.00
In Stock
Gene Symbol:
NSF
Official Gene Full Name:
N-ethylmaleimide sensitive factor, vesicle fusing ATPase
NCBI Gene Id:
60355
Alias Symbols:
SKD2, N-ethylmaleimide-sensitive factor-like protein|NEM-sensitive fusion protein|vesicle-fusing ATPase|vesicular-fusion protein NSF
Description of Target:
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
Protein Name:
Vesicle-fusing ATPase
Swissprot Id:
Q9QUL6
Protein Accession #:
NP_068516.1
Nucleotide Accession #:
NM_021748.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates
Sensitivity:
5.8 pg/mL
Kit Range:
23.5 - 1,500 pg/mL
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology Nsf ELISA Kit (Human) (OKCA01589) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nsf has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nsf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Nsf captured in the well.
Kit Component:
ComponentAmount
Nsf Microplate96 Wells (12 x 8 Well strips)
Nsf Lyophilized Standard2 x 1.5 ng
100X Biotinylated Nsf Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat8480-92
1:2105100-110
1:49085-94
1:89792-101
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9590-100
EDTA Plasma (n=4)8682-90
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Nsf.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Nsf.
Datasheets / Downloads:
Printable datasheet for OKCA01589
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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