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Nrn1 ELISA Kit (Mouse) (OKEH04625)

96 Wells
$606.00
In Stock
Gene Symbol:
NRN1
Official Gene Full Name:
Neuritin 1
NCBI Gene Id:
68404
Alias Symbols:
0710008J23Rik, Candidate plasticity gene 15 protein, cpg15, Cpg15, MGC40786, Neuritin, Nrn
Description of Target:
Promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells.
Protein Name:
Neuritin
Swissprot Id:
Q8CFV4
Protein Accession #:
NP_705757.1
Nucleotide Accession #:
NM_153529.2
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 1.95 ng/mL
Kit Range:
7.8 - 500 pg/mL
Kit Reproducibility:
Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology Nrn1 ELISA Kit (Mouse) (OKEH04625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nrn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Nrn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nrn1 captured in well.
Kit Component:
ComponentAmount
Anti-Nrn1 Microplate96 Wells (12 x 8 Well Strips)
Nrn1 Lyophilized Standard2
Sample Diluent1 x 20 mL
Nrn1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NRN1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NRN1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural mouse FDP. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
15 KDa
Protein Size (# AA):
142
Protocol:
Tips Information:

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