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NPVF ELISA Kit (Human) (OKCA01242)

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96 Wells
$600.00
In Stock
Gene Symbol:
NPVF
Official Gene Full Name:
neuropeptide VF precursor
NCBI Gene Id:
64111
Alias Symbols:
tcag7.1077, C7orf9, RFRP, FMRFamide-related peptides|RFamide-related peptide|neuropeptide NPVF
Description of Target:
Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia.
Protein Name:
Pro-FMRFamide-related neuropeptide VF
Swissprot Id:
Q9HCQ7
Protein Accession #:
NP_071433.3
Nucleotide Accession #:
NM_022150.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
7.81 pg/mL
Kit Range:
31.25 - 2,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01242 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01242 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology NPVF ELISA Kit (Human) (OKCA01242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPVF has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPVF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPVF captured in the well.
Kit Component:
ComponentAmount
NPVF Microplate96 Wells (12 x 8 Well strips)
NPVF Lyophilized Standard2 x 2 ng
100X Biotinylated NPVF Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat10297-106
1:28681-92
1:49489-98
1:810396-107
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)8982-93
EDTA Plasma (n=4)10397-106
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NPVF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NPVF.
Datasheets/Manuals:
Printable datasheet/reference manual for OKCA01242
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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