NPVF ELISA Kit (Human) (OKCA01242)

Catalog#: OKCA01242

• Size: 96 Wells

NPVF ELISA Standard Curve

• Gene Symbol: NPVF
• Official Gene Full Name: neuropeptide VF precursor
• NCBI Gene Id: 64111
• Alias Symbols: tcag7.1077, C7orf9, RFRP, FMRFamide-related peptides|RFamide-related peptide|neuropeptide NPVF
• Description of Target: Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia.
• Protein Name: Pro-FMRFamide-related neuropeptide VF
• Swissprot Id: Q9HCQ7
• Protein Accession #: NP_071433.3
• Nucleotide Accession #: NM_022150.3
• Species Reactivity: Human
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.81 pg/mL
• Kit Range: 31.25 - 2,000 pg/mL
• Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01242 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01242 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• Kit Duration: ~ 3.5 Hours
• Kit Principle: Aviva Systems Biology NPVF ELISA Kit (Human) (OKCA01242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPVF has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPVF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPVF captured in the well.
• Kit Component:

  Component	Amount
  NPVF Microplate	96 Wells (12 x 8 Well strips)
  NPVF Lyophilized Standard	2 x 2 ng
  100X Biotinylated NPVF Detector Anitbody	1 x 120 uL
  100X Avidin-HRP Conjugate	1 x 120 uL
  Sample Diluent	1 x 50 mL
  Detector Antibody Diluent	1 x 15 mL
  Conjugate Diluent	1 x 15 mL
  25X Wash Buffer	1 x 20 mL
  Stop Solution	1 x 10 mL
  TMB Substrate	1 x 10 mL

• Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	102	97-106
  1:2	86	81-92
  1:4	94	89-98
  1:8	103	96-107

• Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	89	82-93
  EDTA Plasma (n=4)	103	97-106

• Kit Detection Method: Colorimetric, OD450 nm
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express NPVF.
• Datasheets/Manuals: Printable datasheet/reference manual for OKCA01242
• Reconstitution and Storage: Store at 2 - 8C. Do not use the kit beyond the expiration date.
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Protocol:

Tips Information: