Now Offering Over 102,157 Antibodies & 44,722 Antigens!

NONO antibody - N-terminal region (ARP40715_T100)

100 ul

Regular Price: $229.00

Special Price: $215.00

In Stock

Conjugation Options

ARP40715_T100-FITC Conjugated

ARP40715_T100-HRP Conjugated

ARP40715_T100-Biotin Conjugated

Gene Symbol:
NONO
NCBI Gene Id:
4841
Official Gene Full Name:
Non-POU domain containing, octamer-binding
Protein Name:
Non-POU domain-containing octamer-binding protein
Swissprot Id:
Q15233
Protein Accession #:
NP_031389
Nucleotide Accession #:
NM_007363
Alias Symbols:
P54, NMT55, NRB54, P54NRB
Replacement Item:
This antibody may replace item sc-23247 from Santa Cruz Biotechnology.
Description of Target:
NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
Protein Size (# AA):
471
Molecular Weight:
52kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Protein A purified
Application:
IF, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NONO.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NONO.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human NONO
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
Complete computational species homology data:
Anti-NONO (ARP40715_T100)
Peptide Sequence:
Synthetic peptide located within the following region: KQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLK
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
SFPQ; PTBP1; PRKAA2; PIN1; NONO; FUS; DDX6; C11orf68; PSPC1; HUWE1; LMO4; UBC; SUMO2; STAU1; MDM2; RPA3; RPA2; RPA1; ASB18; WWOX; ASB3; ERG; EED; rev; WBP4; TCERG1; APBB1; PRPF40A; LMNA; CD81; PAN2; CTNNB1; ORC5; MYC; ITGA4; IL7R; FN1; EWSR1; YLPM1; RNF43
Blocking Peptide:
For anti-NONO (ARP40715_T100) antibody is Catalog # AAP40715 (Previous Catalog # AAPY00967)
Datasheets/Manuals:
Printable datasheet for anti-NONO (ARP40715_T100) antibody
Target Reference:
Kimura,K., (2006) Genome Res. 16 (1), 55-65
Publications:

Bubenik, J. L., Ladd, A. N., Gerber, C. A., Budiman, M. E. & Driscoll, D. M. Known turnover and translation regulatory RNA-binding proteins interact with the 3’ UTR of SECIS-binding protein 2. RNA Biol. 6, 73-83 IF, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat 19106619

Tell us what you think about this item!

Write A Review
    Please, wait...