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NBL1 ELISA Kit (Human) (OKCD00633)

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96 Wells
$585.00
In Stock
Application:
ELISA
Gene Symbol:
NBL1
Official Gene Full Name:
neuroblastoma 1, DAN family BMP antagonist
NCBI Gene Id:
100532736
Alias Symbols:
D1S1733E, DAN, DAND1, DAN domain family member 1, MGC8972, NB, Neuroblastoma suppressor of tumorigenicity 1, NO3, Protein N03, Zinc finger protein DAN
Description of Target:
Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
Protein Name:
neuroblastoma suppressor of tumorigenicity 1
Swissprot Id:
P41271
Protein Accession #:
NP_001191013
Nucleotide Accession #:
NM_001204084.2
Species Reactivity:
Human
Sample Type:
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
< 0.059 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level NBL1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level NBL1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology NBL1 ELISA Kit (Human) (OKCD00633) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NBL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NBL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NBL1captured in well.
Kit Component:
ComponentAmount
Anti-NBL1 Microplate96 Wells (12 x 8 Well Strips)
NBL1 Lyophilized Standard2 x 10 ng
100X Biotinylated NBL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NBL1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NBL1.
Datasheets / Downloads:
Printable datasheet for OKCD00633
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1). No significant cross-reactivity or interference between Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
181
Protocol:
Tips Information:
 
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