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MVP ELISA Kit (Human) (OKEH04434)

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96 Wells
$596.00
In Stock
Gene Symbol:
MVP
Official Gene Full Name:
major vault protein
NCBI Gene Id:
9961
Alias Symbols:
Cyclophilin B, CYPB, CYP-S1, MGC14109, MGC2224, OI9, Peptidyl-prolyl cis-trans isomerase B, PPIase B, Rotamase B, S-cyclophilin, SCYLP
Description of Target:
This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.
Protein Name:
Major vault protein
Swissprot Id:
Q14764
Protein Accession #:
NP_001280133.1
Nucleotide Accession #:
NM_001293204.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.07 ng/mL
Kit Range:
0.15 - 10 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 5.4% (n = 20)
Mean Inter-assay CV%: <= 9.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MVP ELISA Kit (Human) (OKEH04434) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MVP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for MVP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MVP captured in the well.
Kit Component:
ComponentAmount
Anti-MVP Microplate96 Wells (12 x 8 Well Strips)
MVP Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
MVP Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MVP.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MVP.
Datasheets / Downloads:
Printable datasheet for OKEH04434
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human MVP. No significant cross-reactivity or interference was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
99 KDa
Protein Size (# AA):
893
Protocol:
Tips Information:
 
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