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MUC15 ELISA Kit (Human) (OKEH04436)

96 Wells
$606.00
In Stock
Gene Symbol:
MUC15
Official Gene Full Name:
mucin 15, cell surface associated
NCBI Gene Id:
143662
Alias Symbols:
Csfg, G-CSF, Granulocyte colony-stimulating factor, MGI-IG
Description of Target:
May play a role in the cell adhesion to the extracellular matrix.
Protein Name:
Mucin-15
Swissprot Id:
Q8N387
Protein Accession #:
NP_001128563.1
Nucleotide Accession #:
NM_001135091.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.15ng/mL
Kit Range:
0.31 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MUC15 ELISA Kit (Human) (OKEH04436) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUC15 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for MUC15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUC15 captured in the well.
Kit Component:
ComponentAmount
Anti-MUC15 Microplate96 Wells (12 x 8 Well Strips)
MUC15 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
MUC15 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MUC15.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MUC15.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural human MUC-15. No significant cross-reactivity or interference was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
36 KDa
Protein Size (# AA):
334
Protocol:
Tips Information:

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