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MMP-9 ELISA Kit (Human) (OKBB00226)

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96 Tests
$425.00
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Application:
ELISA
Gene Symbol:
MMP-9
Official Gene Full Name:
matrix metallopeptidase 9
NCBI Gene Id:
4318
Alias Symbols:
Matrix metalloproteinase-9, MMP9, CLG4B
Description of Target:
The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
Protein Name:
Matrix metalloproteinase-9
Swissprot Id:
P14780
Protein Accession #:
NP_004985.2
Nucleotide Accession #:
NM_004994.2
Species Reactivity:
Human
Sample Type:
cell culture supernates, serum, plasma(heparin), saliva and urine
Sensitivity:
< 5 pg/mL
Kit Range:
156 pg/mL-10,000 pg/mL
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (ng/mL)1.353.86.91.73.97.1
St. Dev.0.0420.160.240.0950.3080.511
%CV3.114.213.475.587.897.19
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MMP-9 ELISA Kit (Human) (OKBB00226) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for MMP-9 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, A20-D707) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP-9 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human MMP-9 captured in well.
Kit Component:
ComponentAmount
Lyophilized recombinant human MMP-7 standard10 ng/tube x 2
Anti-human MMP-7 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- human MMP-7 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MMP-9.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MMP-9.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine.
Datasheets / Downloads:
OKBB00226
Reconstitution and Storage:
Store at 4C for frequent use, at  -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Target Reference:
1. Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.; Gerhard, D. S. On the structure and chromosome location of the 72-
and 92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991.
2. Turner, H. E.; Nagy, Z.; Esiri, M. M.; Harris, A. L.; Wass, J. A. H. Role of matrix metalloproteinase 9 in pituitary tumor
behavior. J. Clin. Endocr. Metab. 85: 2931-2935, 2000.
3. Zhang, B.; Ye, S.; Herrmann, S.-M.; Eriksson, P.; de Maat, M.; Evans, A.; Arveiler, D.; Luc, G.; Cambien, F.;
Hamsten, A.; Watkins, H.; Henney, A. M. Functional polymorphism in the regulatory region of gelatinase B gene in
relation to severity of coronary atherosclerosis. Circulation 99: 1788-1794, 1999.
4. Coussens, L. M.; Tinkle, C. L.; Hanahan, D.; Werb, Z. MMP-9 supplied by bone marrow-derived cells contributes to
skin carcinogenesis. Cell 103: 481-490, 2000.
5. Minematsu, N.; Nakamura, H.; Tateno, H.; Nakajima, T.; Yamaguchi, K. Genetic polymorphism in matrix
metalloproteinase-9 and pulmonary emphysema. Biochem. Biophys. Res. Commun. 289: 116-119, 2001.
Specificity:
There is no detectable cross-reactivity with other relevant proteins.
Additional Information:
Range: 156pg/ml-10,000pg/ml
:::
Principle: Aviva’s human MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-9 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-9 amount of sample captured in plate.
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
Protocol:
Tips Information:
 
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