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MDC ELISA Kit (Human) (OKBB00213)

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96 Tests
$370.00
In Stock
Application:
ELISA
Gene Symbol:
CCL22
Official Gene Full Name:
C-C motif chemokine ligand 22
NCBI Gene Id:
6367
Alias Symbols:
CCL22, MDC, SCYA22, C-C motif chemokine 22
Description of Target:
Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.
Protein Name:
C-C motif chemokine 22
Swissprot Id:
O00626
Protein Accession #:
NP_002981.2
Nucleotide Accession #:
NM_002990.4
Species Reactivity:
Human
Sample Type:
cell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity:
< 1 pg/mL
Kit Range:
31.2 pg/mL-2,000 pg/mL
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)17872613222429971634
St. Dev.8.238.58212.163.81114.4
%CV4.605.306.2056.407.00
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CCL22 ELISA Kit (Human) (OKBB00213) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for CCL22 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (E.coli, G25-Q93) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL22 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human CCL22 captured in well.
Kit Component:
ComponentAmount
Lyophilized recombinant human IL-12(p40) standard10 ng/tube x 2
Anti-human IL-12(p40) Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- human IL-12(p40) antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCL22.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCL22.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine
Datasheets / Downloads:
OKBB00213
Reconstitution and Storage:
Store at 4C for frequent use, at -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Target Reference:
1. Imai, T.; Chantry, D.; Raport, C. J.; Woods, C. L.; Nishimura, M.; Godiska, R.; Yoshie, O.; Gray, P. W. :
Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J. Biol. Chem. 273:
1764-1768, 1998.
2. Godiska, R.; Chantry, D.; Raport, C. J.; Sozzani, S.; Allavena, P.; Leviten, D.; Mantovani, A.; Gray, P. W. : Human
macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and
natural killer cells. J. Exp. Med. 185: 1595-1604, 1997.
Specificity:
There is no detectable cross-reactivity with other relevant proteins.
Additional Information:
Range: 31.2pg/ml-2000pg/ml
:::
Principle: Aviva’s human MDC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MDC specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MDC amount of sample captured in plate.
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene.1 MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.2
Protocol:
Tips Information:
 
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