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Malachite green ELISA Kit (OKAO00134)

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96 Wells
$470.00
In Stock
Alias Symbols:
MG, MALACHITE GREEN, Basic Green 4, Victoria Green B, Malachite green chloride, Aniline Green, Grenoble Green
Description of Target:
C.i. basic green 4 is a green crystals with metallic luster. Water solutions are blue-green. Used in dyeing silk, wool, biological staining, etc.
Protein Name:
Malachite green
Species Reactivity:
General
Sample Type:
Tissue (fish, shrimp)
Sensitivity:
ComponentAmount
Tissue0.2 ppb
Kit Range:
0.5-8 ppb
Kit Reproducibility:
Mean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology Malachite green ELISA Kit (OKAO00134) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Malachite green has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Malachite green antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
Kit Component:
ComponentAmount
Malachite green Microplate96 Wells (12 x 8 Well Strips)
Malachite green Standards6 x 1 mL
Malachite green Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 0.7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 15 mL
Sample Prep Buffer5 x 20 mL
Kit Recovery:
ComponentAmount
Tissue85% +/- 20%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
SubstanceCross Reactivity Rate
Malachite green100%
Leucomalachite green100%
Assay Info:
Assay Methodology: Competitive Inhibition ELISA
Molecular Weight:
364.911 g/mol
Additional Information:
CAS: 569-64-2
PubChem: 11294
:::
Molecular Formula: C23H25ClN2
Protocol:
Tips Information:
 
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