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LIPA ELISA Kit (Mouse) (OKCD00901)

96 Wells
$565.00
In Stock
Application:
ELISA
Gene Symbol:
LIPA
Official Gene Full Name:
lysosomal acid lipase A
NCBI Gene Id:
16889
Alias Symbols:
AA960673, Acid cholesteryl ester hydrolase, Cholesteryl esterase, Lal, LAL, Lip1, Lip-1, Lipase A, Lysosomal acid lipase/cholesteryl ester hydrolase, Sterol esterase
Description of Target:
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation (By similarity).By similarity
Protein Name:
lysosomal acid lipase/cholesteryl ester hydrolase
Swissprot Id:
Q9Z0M5
Protein Accession #:
NP_001104570
Nucleotide Accession #:
NM_001111100.1
Species Reactivity:
Mouse
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
< 0.126 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Lipa were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Lipa were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Lipa ELISA Kit (Mouse) (OKCD00901) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lipa has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lipa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Lipacaptured in well.
Kit Component:
ComponentAmount
Anti-Lipa Microplate96 Wells (12 x 8 Well Strips)
Lipa Lyophilized Standard2 x 20 ng
100X Biotinylated Lipa Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
erum(n=5)93-101%79-93%96-104%87-105%
EDTA plasma(n=5)97-105%90-97%78-104%98-105%
heparin plasma(n=5)84-97%94-101%80-94%83-104%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10299
EDTA plasma(n=5)88-10394
heparin plasma(n=5)80-9386
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LIPA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LIPA.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Lipase A, Lysosomal Acid (LIPA). No significant cross-reactivity or interference between Lipase A, Lysosomal Acid (LIPA) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
397
Protocol:
Tips Information:

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