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LILRB1 ELISA Kit (Human) : 96 Wells (OKEH01927)

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96 Wells
$450.00
In Stock
Gene Symbol:
LILRB1
Official Gene Full Name:
leukocyte immunoglobulin like receptor B1
NCBI Gene Id:
10859
Alias Symbols:
CD85, CD85 antigen-like family member J, CD85j, CD85J, FLJ37515, ILT2, ILT-2, Immunoglobulin-like transcript 2, Leukocyte immunoglobulin-like receptor 1, Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR1, LIR-1, MIR7, MIR-7, Monocyte/macro
Description of Target:
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Protein Name:
Leukocyte immunoglobulin-like receptor subfamily B member 1
Swissprot Id:
Q8NHL6
Protein Accession #:
NP_001075106.2
Nucleotide Accession #:
NM_001081637.2
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 10 pg/mL
Kit Range:
31.2 - 2000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 10.5% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology LILRB1 ELISA Kit (Human) (OKEH01927) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LILRB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LILRB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LILRB1 captured in well.
Kit Component:
ComponentAmount
LILRB1 Microplate96 Wells (12 x 8 Well strips)
LILRB1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HLA-DRB1-15 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express LILRB1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express LILRB1.
Datasheets / Downloads:
Printable datasheet for OKEH01927
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural humanLILRB1. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
71 kDa
Protein Size (# AA):
650
Protocol:
Tips Information:
 
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