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ITGA3 ELISA Kit (Mouse) (OKCD00825)

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96 Wells
$535.00
In Stock
Application:
ELISA
Gene Symbol:
ITGA3
Official Gene Full Name:
integrin alpha 3
NCBI Gene Id:
16400
Alias Symbols:
AA407068, alpha3-integrin, CD49 antigen-like family member C, CD49C, Galactoprotein B3, GAPB3, Integrin alpha-3, VLA-3 alpha 3, VLA-3 subunit alpha
Description of Target:
Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.By similarity <p>Manually curated information which has been propagated from a related experimentally characterized protein.</p> <p><a href="/manual/evidences#ECO:0000250">More…</a></p> Manual assertion inferred from sequence similarity toiUniProtKB:P26006 (ITA3_HUMAN)
Protein Name:
integrin alpha-3
Swissprot Id:
Q62470
Protein Accession #:
NP_001293000
Nucleotide Accession #:
NM_001306071.1
Species Reactivity:
Mouse
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
< 0.118 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Itga3 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Itga3 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Itga3 ELISA Kit (Mouse) (OKCD00825) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Itga3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Itga3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Itga3captured in well.
Kit Component:
ComponentAmount
Anti-Itga3 Microplate96 Wells (12 x 8 Well Strips)
Itga3 Lyophilized Standard2 x 20 ng
100X Biotinylated Itga3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ITGA3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ITGA3.
Datasheets / Downloads:
Printable datasheet for OKCD00825
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 3 (ITGa3). No significant cross-reactivity or interference between Integrin Alpha 3 (ITGa3) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
1053
Protocol:
Tips Information:
 
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