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IKBKB ELISA Kit (Rat) : 96 Wells (OKEH01391)

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96 Wells
$420.00
In Stock
Gene Symbol:
IKBKB
Official Gene Full Name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
NCBI Gene Id:
84351
Alias Symbols:
AIM-1, I-kappa-B kinase 2, I-kappa-B-kinase beta, IkBKB, IKK2, Ikkb, IKK-B, IKK-beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta
Description of Target:
kinase that may be involved in megakaryocyte polyploidization
Protein Name:
Inhibitor of nuclear factor kappa-B kinase subunit beta
Swissprot Id:
Q9QY78
Protein Accession #:
NP_445807.2
Nucleotide Accession #:
NM_053355.2
Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.12 ng/ml
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology IKBKB ELISA Kit (Rat) (OKEH01391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IKBKB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IKBKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IKBKB captured in well.
Kit Component:
ComponentAmount
IKBKB Microplate96 Wells (12 x 8 Well strips)
IKBKB Lyophilized Standard2
100X Biotinylated IKBKB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IKBKB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IKBKB.
Datasheets / Downloads:
Printable datasheet for OKEH01391
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural rat I-kappa-B-kinase beta. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
87 kDa
Protein Size (# AA):
757
Protocol:
Tips Information:
 
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