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Human IgG (gamma chain) Antibody - HRP Conjugated (OARA04964)

1mg
$370.00
In Stock
Description of Target:
Anti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain). It is a protein complex composed of four peptide chain - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG (gamma chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Molecular Weight:
40 kDa (label M/w)
Host:
Goat
Clonality:
Polyclonal
Purification:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Application:
ELISA, WB, IHC
Immunogen:
Human IgG gamma chain
Species Reactivity:
Human
Product Format:
Lyophilized 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2), 10 mg/mL BSA (immunoglobulin & protease free) with 0.01% (w/v) Gentamicin Sulfate
Color: Substrate dependent
Reconstitution and Storage:
Reconstitute with 1.0 mL of deionized water (or equivalent). Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Do NOT add sodium azide!
Concentration:
1.0 mg/ml (by UV absorbance at 280 nm)
Datasheets/Manuals:
Printable datasheet for OARA04964
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Conjugation:
HRP
Isotype:
IgG
Application Info:
ELISA 1:10,000~100,000
WB 1:500~1,000
IHC 1:2,000~10,000
Anti-Human IgG Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

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