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HSPA8 ELISA Kit (Human) (OKEH04446)

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96 Wells
$596.00
In Stock
Gene Symbol:
HSPA8
Official Gene Full Name:
heat shock protein family A (Hsp70) member 8
NCBI Gene Id:
3312
Alias Symbols:
A030005E02Rik, AI324033, Lap, PAP, Ppal, Prostatic acid phosphatase
Description of Target:
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Protein Name:
Heat shock cognate 71 kDa protein
Swissprot Id:
P11142
Protein Accession #:
NP_006588.1
Nucleotide Accession #:
NM_006597.5
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.15ng/mL
Kit Range:
0.31 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 6.1% (n = 20)
Mean Inter-assay CV%: <= 9.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology HSPA8 ELISA Kit (Human) (OKEH04446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for HSPA8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA8 captured in the well.
Kit Component:
ComponentAmount
Anti-HSPA8 Microplate96 Wells (12 x 8 Well Strips)
HSPA8 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
HSPA8 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HSPA8.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HSPA8.
Datasheets / Downloads:
Printable datasheet for OKEH04446
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human Heat shock cognate 71 kDa protein. No significant cross-reactivity or interference was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
71 KDa
Protein Size (# AA):
646
Protocol:
Tips Information:
 
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