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GPX1 ELISA Kit (Human) : 96 Wells (OKEH00367)

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96 Wells
$420.00
In Stock
Gene Symbol:
GPX1
Official Gene Full Name:
glutathione peroxidase 1
NCBI Gene Id:
2876
Alias Symbols:
Cellular glutathione peroxidase, Glutathione peroxidase 1, GPx-1, GSHPX1, GSHPx-1, MGC14399, MGC88245
Description of Target:
Protects the hemoglobin in erythrocytes from oxidative breakdown.
Protein Name:
Glutathione peroxidase 1
Swissprot Id:
P07203
Protein Accession #:
NP_000572.2
Nucleotide Accession #:
NM_000581.2
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 3.12 U/mL
Kit Range:
12.5 - 800 U/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology GPX1 ELISA Kit (Human) (OKEH00367) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPX1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for GPX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPX1 captured in well.
Kit Component:
ComponentAmount
GPX1 Microplate96 Wells (12 x 8 Well strips)
GPX1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GPX1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GPX1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GPX1.
Datasheets / Downloads:
Printable datasheet for OKEH00367
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human GPx-1. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
22 kDa
Protein Size (# AA):
203
Protocol:
Tips Information:
 
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