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GPR37 ELISA Kit (Human) (OKDD00293)

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96 Wells
$580.00
In Stock
Gene Symbol:
GPR37
Official Gene Full Name:
G protein-coupled receptor 37
NCBI Gene Id:
2861
Alias Symbols:
EDNRBL, Endothelin B receptor-like protein 1, ETBR-LP-1, hET(B)R-LP, PAELR, Parkin-associated endothelin receptor-like receptor
Description of Target:
This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease.
Protein Name:
prosaposin receptor GPR37
Swissprot Id:
O15354
Protein Accession #:
NP_005293.1
Nucleotide Accession #:
NM_005302.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell lysates
Sensitivity:
< 0.057 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GPR37 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GPR37 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology GPR37 ELISA Kit (Human) (OKDD00293) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for GPR37 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPR37 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GPR37 captured in well.
Kit Component:
ComponentAmount
Anti-GPR37 Microplate96 Wells (12 x 8 Well Strips)
GPR37 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X GPR37 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GPR37.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GPR37.
Datasheets / Downloads:
Printable datasheet for OKDD00293
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of GPR37. No significant cross-reactivity or interference between GPR37 and analogues was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
67 kDa
Protein Size (# AA):
613
Protocol:
Tips Information:
 
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