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FZD3 ELISA Kit (Human) (OKEH02139)

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96 Wells
$420.00
In Stock
Application:
ELISA
Gene Symbol:
FZD3
Official Gene Full Name:
frizzled class receptor 3
NCBI Gene Id:
7976
Alias Symbols:
Frizzled-3, Fz-3, hFz3
Description of Target:
This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia.
Protein Name:
Frizzled-3
Swissprot Id:
Q9NPG1
Protein Accession #:
NP_059108.1
Nucleotide Accession #:
NM_017412.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.23 ng/mL
Kit Range:
0.78 - 50 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology FZD3 ELISA Kit (Human) (OKEH02139) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FZD3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FZD3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FZD3 captured in well.
Kit Component:
ComponentAmount
FZD3 Microplate96 Wells (12 x 8 Well strips)
FZD3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FZD3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express FZD3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express FZD3.
Datasheets / Downloads:
Printable datasheet for OKEH02139
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human Fz-3. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
76 kDa
Protein Size (# AA):
666
Protocol:
Tips Information:
 
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