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FKBPL ELISA Kit (Mouse) (OKCD01069)

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96 Wells
$600.00
In Stock
Application:
ELISA
Gene Symbol:
FKBPL
Official Gene Full Name:
FK506 binding protein-like
NCBI Gene Id:
56299
Alias Symbols:
DIR1, FK506-binding protein-like, Ng7, NG7, Ppiase-X, WAF-1/CIP1 stabilizing protein 39, Wisp39, WISp39
Description of Target:
May be involved in response to X-ray. Regulates p21 protein stability by binding to Hsp90 and p21.By similarity
Protein Name:
FK506-binding protein-like
Swissprot Id:
O35450
Protein Accession #:
NP_063926
Nucleotide Accession #:
NM_019873.2
Species Reactivity:
Mouse
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
< 0.113 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Fkbpl were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Fkbpl were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Fkbpl ELISA Kit (Mouse) (OKCD01069) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fkbpl has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fkbpl is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Fkbplcaptured in well.
Kit Component:
ComponentAmount
Anti-Fkbpl Microplate96 Wells (12 x 8 Well Strips)
Fkbpl Lyophilized Standard2 x 20 ng
100X Biotinylated Fkbpl Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express FKBPL.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express FKBPL.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein Like Protein (FKBPL). No significant cross-reactivity or interference between FK506 Binding Protein Like Protein (FKBPL) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
347
Protocol:
Tips Information:
 
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