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FHIT ELISA Kit (Human) (OKCA01246)

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96 Wells
In Stock
Gene Symbol:
Official Gene Full Name:
fragile histidine triad
NCBI Gene Id:
Alias Symbols:
AP3Aase, FRA3B, AP3A hydrolase|bis(5 -adenosyl)-triphosphatase|diadenosine 5 ,5 -P1,P3-triphosphate hydrolase|dinucleosidetriphosphatase|tumor suppressor protein
Description of Target:
Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca2+ transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
Protein Name:
Swissprot Id:
Protein Accession #:
Nucleotide Accession #:
Species Reactivity:
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
7.8 pg/mL
Kit Range:
31.2 - 2,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01246 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01246 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology FHIT ELISA Kit (Human) (OKCA01246) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FHIT has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FHIT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FHIT captured in the well.
Kit Component:
FHIT Microplate96 Wells (12 x 8 Well strips)
FHIT Lyophilized Standard2 x 2 ng
100X Biotinylated FHIT Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9691-100
EDTA Plasma (n=4)10196-107
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express FHIT.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express FHIT.
Datasheets / Downloads:
Printable datasheet for OKCA01246
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Tips Information:
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