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FGF2 ELISA Kit (Human) : 48 Wells (OKAA00030_48W)

  • Catalog#: OKAA00030_48W
  • 2 weeks
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48 Wells
In Stock
Gene Symbol:
Official Gene Full Name:
fibroblast growth factor 2
NCBI Gene Id:
Alias Symbols:
Basic fibroblast growth factor, bFGF, BFGF, FGFB, HBGF-2, Heparin-binding growth factor 2
Description of Target:
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Protein Name:
Fibroblast growth factor 2
Swissprot Id:
Protein Accession #:
Nucleotide Accession #:
Species Reactivity:
Sample Type:
cell culture, supernates, serum, and plasma
7 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
2 vials human FGF-basic Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human FGF-basic antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express FGF2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express FGF2.
Predicted Homology Based on Immunogen Sequence:
Datasheets / Downloads:
This assay recognizes both natural and recombinant human VEGF. The factors listed below were prepared at 200 ng/ml in Standard/ sample diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: EC-VEGF/PK1, PDGF-AA, PDGF-AB , PDGF-BB, PDGF
Recombinant mouse: PDGF-2, VEGF120, VEGF164, VEGF R2(KDR)/Fc Chimera
Recombinant Rat: VEGF 120(0.07%)
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
Reagents: REAGENTS
1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human FGF-basic (8x12)
2. 2 vials human FGF-basic Standard lyophilized, 1000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human FGF-basic monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Tips Information:
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