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FAS-L ELISA Kit (Rat) (OKBB00494)

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96 Wells
$385.00
In Stock
Application:
ELISA
Gene Symbol:
FASLG
Official Gene Full Name:
Tumor necrosis factor ligand superfamily member 6
NCBI Gene Id:
25385
Alias Symbols:
Apt1Lg1, Cd95l, CD95-L, CD95 ligand, Fas antigen ligand, Fasl, Fas ligand, Tnfsf6, Tumor necrosis factor ligand superfamily member 6
Description of Target:
FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
Protein Name:
Tumor necrosis factor ligand superfamily member 6
Swissprot Id:
P36940
Protein Accession #:
NP_037040.1
Nucleotide Accession #:
NM_012908.1
Species Reactivity:
Rat
Sample Type:
Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sensitivity:
<= 10 pg/mL
Kit Range:
31.2 pg/mL-2,000 pg/mL
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)11885413191028471423
SD4.1340.9971.235.252.51103.9
CV%3.54.85.45.16.27.3
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology FAS-L ELISA Kit (Rat) (OKBB00494) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A mouse monoclonal antibody specific for FAS-L has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO; Q127-L278) and test samples are added to the wells and incubated. After washing, abiotinylated polyclonal goat detector antibody specific for FAS-L is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rat FAS-L captured in well.
Kit Component:
ComponentAmount
96-Well plate Pre-coated with Anti-Rat FAS-L Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Rat FAS-L Standard2 x 10 ng/tube
100X Biotinylated Anti-Rat FAS-L Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express FASLG.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express FASLG.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protocol:
Tips Information:
 
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