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FADS2 ELISA Kit (Rat) (OKEH04313)

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96 Wells
$596.00
In Stock
Gene Symbol:
FADS2
Official Gene Full Name:
fatty acid desaturase 2
NCBI Gene Id:
83512
Alias Symbols:
D6D, Delta(6) desaturase, Delta(6) fatty acid desaturase, Delta-6 desaturase, Fadsd6, Fatty acid desaturase 2, MGC93365
Description of Target:
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions.
Protein Name:
fatty acid desaturase 2
Swissprot Id:
Q9Z122
Protein Accession #:
NP_112634.1
Nucleotide Accession #:
NM_031344.2
Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity:
< 0.39 ng/mL
Kit Range:
0.78 - 50 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Fads2 ELISA Kit (Rat) (OKEH04313) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fads2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fads2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fads2 captured in the well.
Kit Component:
ComponentAmount
Fads2 Microplate96 Wells (12 x 8 Well Strips)
Fads2 Standard2 x 2 ng
Sample Diluent1 x 20 mL
Fads2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express FADS2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express FADS2.
Datasheets / Downloads:
Printable datasheet for OKEH04313
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural rat Fatty acid desaturase 2. No significant cross-reactivity or interference was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
52 kDa
Protein Size (# AA):
444
Protocol:
Tips Information:
 
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