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EWSR1 antibody - middle region (ARP40749_P050)

100 ul

Regular Price: $289.00

Special Price: $215.00

In Stock

Conjugation Options

ARP40749_P050-FITC Conjugated

ARP40749_P050-HRP Conjugated

ARP40749_P050-Biotin Conjugated

Gene Symbol:
EWSR1
NCBI Gene Id:
2130
Official Gene Full Name:
Ewing sarcoma breakpoint region 1
Protein Name:
RNA-binding protein EWS
Swissprot Id:
Q01844
Protein Accession #:
NP_005234
Nucleotide Accession #:
NM_005243
Alias Symbols:
EWS, bK984G1.4
Replacement Item:
This antibody may replace item sc-111059 from Santa Cruz Biotechnology.
Description of Target:
EWSR1 is a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.
Protein Size (# AA):
656
Molecular Weight:
68kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express EWSR1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express EWSR1.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human EWSR1
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Complete computational species homology data:
Anti-EWSR1 (ARP40749_P050)
Peptide Sequence:
Synthetic peptide located within the following region: QPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQS
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
SSBP3; SEC24A; TFG; SEC24D; PEF1; MAPK1IP1L; ATPAF2; TRAF2; TRAF1; PLSCR1; PRR13; UBC; RPA3; RPA2; RPA1; EZH2; SUZ12; RNF2; BMI1; rev; ITCH; WBP4; PRMT1; CCDC7; YY1AP1; MRPS18B; UBD; CD81; RIOK2; NONO; ITGB5; ITGA4; FUS; FN1; EWSR1; EPAS1; EP300; HNRNPUL1
Blocking Peptide:
For anti-EWSR1 (ARP40749_P050) antibody is Catalog # AAP40749 (Previous Catalog # AAPY01001)
Datasheets/Manuals:
Printable datasheet for anti-EWSR1 (ARP40749_P050) antibody
Sample Type Confirmation:

EWSR1 is strongly supported by BioGPS gene expression data to be expressed in HeLa

EWSR1 is supported by BioGPS gene expression data to be expressed in HEK293

Target Reference:
Brandal,P., (2008) Genes Chromosomes Cancer 47 (7), 558-564
Average Rating:
2 reviews
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2 Item(s)

02/01/2017 16:23
  • Overall Experience:
  • Quality:
Product Review: EWSR1 antibody - middle region (ARP40749_P050) in Human pancreatic cancer cell line MiaPaca-2 and Panc-1 using Western Blot
Product Page for EWSR1 antibody - middle region (ARP40749_P050)

Researcher: Dr. Crissy Dudgeon, Ph.D., CINJ
Application: Western blotting
Species + Tissue/Cell type: Human pancreatic cancer cell line MiaPaca-2 and Panc-1
How many ug's of tissue/cell lysate run on the gel:
1. 30 ug MiaPaca-2 cell lysate
2. 30 ug Panc-1 cell lysate
Primary antibody dilution: 1:1000
Secondary antibody: Goat anti-Rabbit HRP
Secondary antibody dilution: 1:4000



Questionnaire:
How do Aviva's reagents play a role in your experiemental goals? I was looking at the endogenous expression of the protein in order to look at in vivo binding to my protein of interest.
How would you rate this antibody on a scale from 1-5 (5=best) and why? 4. It only has one other non-specific band. Worked very well in western blotting.
Would you use this antibody in future experiments? Yes
Have you used another antibody which has worked in your application? No
Do you believe the information about the reagent on Aviva's website is correct? Yes
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Yes, it was a good antibody and detected a specific band.
How did you store the antibody after re-suspension? I put it at 4 C.
Sample Description, Species, Tissue/Cell Type: Homo sapiens, Pancreatic cancer cell line, 30 ug
How many different experimental trials were conducted using the antibody sample? 2
What type of experimental sample are you using and how did you preparing it? Cells were grown at 70% confluency and harvested directly into Laemmli buffer with beta-mercaptoethanol.
Primary used and dilution: 1:1000, overnight at 4C
Secondary used and dilution: Goat anti-rabbit HRP from Jackson ImmunoResearcj (cat 111-005-003) at 1:4000
What controls were used in your experiment? Please include your positive control: None
Experimental Procedure/Protocols: Cells were harvested directly into Laemmli buffer with beta-mercaptoethanol. ~30 ug total protein was headted at 95C for 10 minutes, and then loaded onto a 4-20% Tris Glycine NuPAGE gel. Following PAGE, the the gel was transferred onto Immunobilon-P. Blots were blocked in 5% milk in TBST for 1 hour, then probed overnight in primary antibody at 1:1000. Following 3x washing in TBST 10 minutes each, blots were probed with secondary antibody at 1:4000 for 1h at room temperature. Following washing as before, blots were incubated with ECL plus Western Lightning Solution (PerkinElmer) and exposed to flim
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02/01/2017 16:23
  • Overall Experience:
  • Quality:
Product Review: EWSR1 antibody-middle region (ARP40749_P050) in Hela cell lysate using Western blot
Product Page for EWSR1 antibody - middle region (ARP40749_P050)

Researcher: Archa Fox, University of Western Australia
Application: Western blotting
Species + Tissue/Cell type: Lane 1: 50ug Hela Lysate
Primary antibody dilution: 1:1000
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:10,000


Questionnaire:
How would you rate this antibody on a scale from 1-5 (5=best) and why? 4
Would you use this antibody in future experiments? Yes
Have you used another antibody which has worked in your application? Yes
Do you believe the information about the reagent on Aviva's website is correct? Yes
How did you store the antibody after re-suspension? 4 degrees C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): Human HeLa cell extract.
How many different experimental trials were conducted using the antibody sample? One
How was this sample prepared? SDS-PAGE gel loading buffer pipetted onto adherant cells, followed by scraping and DNA removal.
Primary antibody dilution and incubation time: 1:1000, 1hour incubation.
Secondary antibody used and dilution and incubation time: Anti-rabbit peroxidase, 1:10,000, 1 hour incubation.
What controls were used in your experiment (positive/negative)? Positive controls were other Aviva antibodies that displayed clear bands under the same conditions.
Please include your detailed WB Procedure/Protocol here: HeLa cell lysate was loaded onto each lane of a 12-well 4-12% Bis/Tris NUPAGE gel (Life Technologies) and electrophoresed in MOPS buffer (Life Technologies) for 1.5hrs at 150V. Transfer was performed onto Nitrocellulose using the NUPAGE transfer apparatus and tris/glycin buffer. Membranes were blocked in 5% skim milk, PBS, 0.05% tween overnight at 4degrees.Primary antibody was made up in 5% skim milk, PBS, 0.05% tween, incubated for 1 hour, membranewas washed in PBS, 0.05% tween 3 times, followed by secondary antibody incubation in 5% skim milk, PBS, 0.05% tween, then washed in PBS, 0.05% tween 3 times. Secondary antibody was detected by 5 minutes incubation with chemiluminescent substrate (Luminate Forte, Millipore) and capture with a gel-doc system.
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