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Estrone ELISA Kit (OKEH02608)

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96 Wells
$450.00
In Stock
Alias Symbols:
E1, Estrone, E1, Oestrone
Description of Target:
Estrone is relevant to health and disease states because of its conversion to estrone sulfate, a long-lived derivative. Estrone sulfate acts as a reservoir that can be converted as needed to the more active estradiol. Estrone (E1, and also oestrone) is an estrogenic hormone secreted by the ovary as well as adipose tissue.
Protein Name:
Estrone
Species Reactivity:
All
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity:
< 10 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 11.0% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Estrone ELISA Kit (OKEH02608) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Estrone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Estrone and incubated. The Estrone found in the sample or standards competes with the biotinylated Estrone for limited binding sites on the immobilized anti-Estrone antibody. Excess unbound biotinylated Estrone and sample or standard Estrone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Estrone captured in the well and inversely proportional to the amount of Estrone which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-Estrone Microplate96 Wells (12 x 8 Well strips)
Estrone Lyophilized Standard2 x 1,000 pg
Sample Diluent1 x 20 mL
100X Estrone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into Serum and Plasma = 111%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets / Downloads:
Printable datasheet for OKEH02608
Reconstitution and Storage:
Store at 2-8C for 1 month. Store at -20C for up to 12 months. TMB Substrate and Stop Solution must be stored at 2-8C for 6 months.
Assay Info:
Assay Methodology: Quantitative Competitive ELISA
Additional Information:
Target Abbr: E1
Target Name: Estrone
:::
Pubchem: 5870
:::
Chemical formula: C18H22O2
Protocol:
Tips Information:
 
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