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ESR1/ER Antibody - middle region (OAAB09847)

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400ul
$320.00
In Stock
Gene Symbol:
ESR1
NCBI Gene Id:
2099
Protein Name:
Estrogen receptor
Swissprot Id:
P03372
Protein Accession #:
NP_000116.2
Alias Symbols:
ESR1, ESR, NR3A1, Estrogen receptor, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
Replacement Item:
This antibody may replace item sc-102391 from Santa Cruz Biotechnology.
Description of Target:
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight:
66216 Da
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB, IHC, IF
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ESR1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ESR1.
Species Reactivity:
Cow, Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Cow; Human; Mouse; Pig; Rat
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets / Downloads:
OAAB09847
Sample Type Confirmation:

ESR1 is strongly supported by BioGPS gene expression data to be expressed in MCF7

Specificity:
This ESR1/ER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-273 amino acids from the Central region of human ESR1/ER.
Isotype:
Ig
Application Info:
WB 1:100 - 500
IHC 1:10 - 50
IF 1:10 - 50
Additional Information:
Precautions: ESR1/ER Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
:::
Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1
:::
Cellular Location: Isoform 1: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated
Tissue Location: Widely expressed. Isoform 3 is not expressed in the pituitary gland
Protocol:
Tips Information:

Product Protocol: ESR1 antibody tested with human MCF-7 by western blot (OAAB09847)

Please find the protocol used to generate the western blot data for item (OAAB09847) by going here

Product datasheet link: ESR1 antibody (OAAB09847)

ESR1/ER Antibody (Center) (Cat. #OAAB09847) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESR1/ER antibody detected the ESR1/ER protein (arrow).

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's ESR1 antibody (OAAB09847) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: ESR1 antibody tested with human breast carcinoma in immunohistochemistry (IHC) (OAAB09847)

Please go here to see the protocol used for Aviva's ESR1 antibody (OAAB09847)

Product datasheet can be found here: ESR1 antibody (OAAB09847)

IHC information:
ESR1/ER Antibody (Center) (Cat. #OAAB09847) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's ESR1 antibody (OAAB09847) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: ESR1 antibody tested with human uterus in immunohistochemistry (IHC) (OAAB09847)

Please go here to see the protocol used for Aviva's ESR1 antibody (OAAB09847)

Product datasheet can be found here: ESR1 antibody (OAAB09847)

IHC information:
ESR1/ER Antibody (Center) (Cat. #OAAB09847) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's ESR1 antibody (OAAB09847) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
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