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EOMES ELISA Kit (Human) (OKCD01066)

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96 Wells
$545.00
In Stock
Application:
ELISA
Gene Symbol:
EOMES
Official Gene Full Name:
eomesodermin
NCBI Gene Id:
8320
Alias Symbols:
Eomesodermin homolog, T-box brain protein 2, TBR2, TBR-2, T-brain-2
Description of Target:
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.2 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.8"The T-box transcription factor eomesodermin controls CD8 T cell activity and lymph node metastasis in human colorectal cancer."_x005F_x000D_ Atreya I., Schimanski C.C., Becker C., Wirtz S., Dornhoff H., Schnuerer E., Berger M.R., Galle P.R., Herr W., Neurath M.F._x005F_x000D_ Gut 56:1572-1578(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.Ref.9"Homozygous silencing of T-box transcription factor EOMES leads to microcephaly with polymicrogyria and corpus callosum agenesis."_x005F_x000D_ Baala L., Briault S., Etchevers H.C., Laumonnier F., Natiq A., Amiel J., Boddaert N., Picard C., Sbiti A., Asermouh A., Attie-Bitach T., Encha-Razavi F., Munnich A., Sefiani A., Lyonnet S._x005F_x000D_ Nat. Genet. 39:454-456(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN BRAIN DEVELOPMENT, DEVELOPMENTAL STAGE.
Protein Name:
eomesodermin homolog
Swissprot Id:
O95936
Protein Accession #:
NP_001265111
Nucleotide Accession #:
NM_001278182.1
Species Reactivity:
Human
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
< 0.115 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level EOMES were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level EOMES were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology EOMES ELISA Kit (Human) (OKCD01066) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EOMES has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EOMES is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EOMEScaptured in well.
Kit Component:
ComponentAmount
Anti-EOMES Microplate96 Wells (12 x 8 Well Strips)
EOMES Lyophilized Standard2 x 20 ng
100X Biotinylated EOMES Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express EOMES.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express EOMES.
Datasheets / Downloads:
Printable datasheet for OKCD01066
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Eomesodermin (EOMES). No significant cross-reactivity or interference between Eomesodermin (EOMES) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
686
Protocol:
Tips Information:
 
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