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Enrofloxacin ELISA Kit (OKAO00116)

96 Wells
$370.00
In Stock
Alias Symbols:
ENR, Enrofloxacin, Baytril, 93106-60-6, Enrofloxacine, CFPQ, Bay-Vp-2674
Description of Target:
Enrofloxacin (ENR) is a fluoroquinolone antibiotic sold by the Bayer Corporation under the trade name Baytril. Enrofloxacin is currently approved by the FDA for the treatment of individual pets and domestic animals in the United States. In September 2005, the FDA withdrew approval of Baytril for use in water to treat flocks of poultry, as this practice was noted to promote the evolution of fluoroquinolone-resistant strains of the bacterium Campylobacter, a human pathogen.
Protein Name:
Enrofloxacin
Species Reactivity:
General
Sample Type:
Tissues (chicken, shrimp, fish, liver, etc) and honey.
Sensitivity:
ComponentAmount
Tissue1 ppb
Honey1 ppb
Kit Range:
0.1-8.1 ppb
Kit Reproducibility:
Mean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology Enrofloxacin ELISA Kit (OKAO00116) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Enrofloxacin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Enrofloxacin antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
Kit Component:
ComponentAmount
Enrofloxacin Microplate96 Wells (12 x 8 Well Strips)
Enrofloxacin Standards6 x 1 mL
Enrofloxacin Rabbit Detector Antibody1 x 7 mL
Anti-Rabbit / HRP Conjugate1 x 7 mL
Detection Reagent A1 x 7 mL
Detection Reagent B1 x 7 mL
Stop Solution1 x 7 mL
20X Wash Buffer2 x 20 mL
2X Sample Prep Buffer3 x 15 mL
Kit Recovery:
ComponentAmount
Tissue85% +/- 10%
Honey85% +/- 10%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
SubstanceCross Reactivity Rate
Enrofloxacin100%
Ciprofloxacin3.8%
Norfloxacin2.4%
Sarafloxacin1.8%
Assay Info:
Assay Methodology: Competitive Inhibition ELISA
Molecular Weight:
359.394683 g/mol
Additional Information:
CAS: 93106-60-6
PubChem: 71188
:::
Molecular Formula: C19H22FN3O3
Protocol:
Tips Information:

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