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EFNA3 ELISA Kit (Human) (OKCD02515)

96 Wells
$555.00
In Stock
Gene Symbol:
EFNA3
Official Gene Full Name:
ephrin A3
NCBI Gene Id:
1944
Alias Symbols:
EFL2, EFL-2, Ehk1-L, EHK1-L, EHK1 ligand, EPH-related receptor tyrosine kinase ligand 3, Ephrin-A3, EPLG3, LERK3, LERK-3
Description of Target:
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Protein Name:
Ephrin-A3
Swissprot Id:
P52797
Protein Accession #:
NP_004943.1
Nucleotide Accession #:
NM_004952.4
Species Reactivity:
Human
Sample Type:
Tissue homogenates and other biological fluids
Sensitivity:
0.051 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level EFNA3 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level EFNA3 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Duration:
3 Hours
Kit Principle:
Aviva Systems Biology EFNA3 ELISA Kit (Human) (OKCD02515) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EFNA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for EFNA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EFNA3 captured in well.
Kit Component:
ComponentAmount
Anti-EFNA3 Microplate96 Wells (12 x 8 Well strips)
EFNA3 Lyophilized Standard2
100X Biotinylated EFNA3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express EFNA3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express EFNA3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Ephrin A3 (EFNA3). No significant cross-reactivity or interference between Ephrin A3 (EFNA3) and analogues was observed
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protein Size (# AA):
238
Protocol:
Tips Information:

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