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DAPK1 ELISA Kit (Human) (OKDD00222)

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96 Wells
$580.00
In Stock
Gene Symbol:
DAPK1
Official Gene Full Name:
proteasome activator subunit 3
NCBI Gene Id:
1612
Alias Symbols:
DAPK, DAP kinase 1, Death-associated protein kinase 1, DKFZp781I035
Description of Target:
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.
Protein Name:
Death-associated protein kinase 1
Swissprot Id:
P53355
Protein Accession #:
NP_001275658.1
Nucleotide Accession #:
NM_001288729.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell lysates
Sensitivity:
< 0.052 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DAPK1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DAPK1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology DAPK1 ELISA Kit (Human) (OKDD00222) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for DAPK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DAPK1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DAPK1 captured in well.
Kit Component:
ComponentAmount
Anti-DAPK1 Microplate96 Wells (12 x 8 Well Strips)
DAPK1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DAPK1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express DAPK1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express DAPK1.
Datasheets / Downloads:
Printable datasheet for OKDD00222
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of DAPK1. No significant cross-reactivity or interference between DAPK1 and analogues was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
160 kDa
Protein Size (# AA):
1430
Protocol:
Tips Information:
 
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