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CYBB Antibody (OASA02659)

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0.2mg
$440.00
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Gene Symbol:
CYBB
NCBI Gene Id:
1536
Protein Name:
Cytochrome b-245 heavy chain
Swissprot Id:
P04839
Protein Accession #:
NP_000388.2
Alias Symbols:
CGD, NOX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, CYBB
Replacement Item:
This antibody may replace item sc-130543 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CYTOCHROME B245 HEAVY CHAIN
Protein Size (# AA):
570
Host:
Mouse
Clonality:
Monoclonal
Application:
IHC-AFF, ELISA, FC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CYBB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CYBB.
Immunogen:
The immunogen for anti-CYBB antibody: cytochrome B245 solubilised in octyl-beta-glucopyranoside
Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets / Downloads:
Printable datasheet for anti-CYBB antibody - OASA02659
Specificity:
CYTOCHROME B245
Clone:
NL7
Isotype:
IgG1
Application Info:
(1) Special conditions apply - see [*]
Application Data:
Application #1: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain . Permeabilised with Leucoperm
Application #2: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain: Alexa Fluor 488 . Permeabilised with Leucoperm
Application #3: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain:FITC . Permeabilised with Leucoperm
Application #4: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain:Alexa Fluor ?647 . Permeabilised with Leucoperm
Additional Information:
Fusion Partners: Spleen cells from immunised BALB/c were fused with cells of the 3PU1 myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
:::
Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Burrit, J. B. et al. (2003) Functional epitope on human neutrophil flavocytochrome b558. J. Immunol. 170: 6082 - 6089
2. Taylor, R. M. et al. (2004) Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit. J. Immunol. 173: 7349 - 7357
3. Taylor, R. M. et al. (2007) Cloning, sequence analysis and confirmation of derived gene sequences for three epitope-mapped monoclonal antibodies against human phagocyte flavocytochrome b. Mol. Immunol. 44: 625 - 637
Protocol:
Tips Information:
 
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