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CXCR7 ELISA Kit (Human) (OKEH01934)

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96 Wells
$480.00
In Stock
Application:
ELISA
Gene Symbol:
CXCR7
Official Gene Full Name:
atypical chemokine receptor 3
NCBI Gene Id:
57007
Alias Symbols:
Chemokine orphan receptor 1, CMKOR1, C-X-C chemokine receptor type 7, CXCR-7, CXC-R7, GPR159, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, RDC1, RDC-1
Description of Target:
This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.
Protein Name:
Atypical chemokine receptor 3
Swissprot Id:
P25106
Protein Accession #:
NP_064707.1
Nucleotide Accession #:
NM_020311.2
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.12 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CXCR7 ELISA Kit (Human) (OKEH01934) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCR7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR7 captured in well.
Kit Component:
ComponentAmount
CXCR7 Microplate96 Wells (12 x 8 Well strips)
CXCR7 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CXCR7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CXCR7.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CXCR7.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural human CXC-R7. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
41 kDa
Protein Size (# AA):
362
Protocol:
Tips Information:
 
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