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CXCL16 ELISA Kit (Mouse) (OKBB00317)

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96 Tests
$405.00
In Stock
Application:
ELISA
Gene Symbol:
CXCL16
Official Gene Full Name:
chemokine (C-X-C motif) ligand 16
NCBI Gene Id:
66102
Alias Symbols:
C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SCYB16, SRPSOX
Description of Target:
Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2.1 These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine.2 CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen.1 Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells.1 CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo.1, 3 Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13.4 The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.
Protein Name:
C-X-C motif chemokine 16
Swissprot Id:
Q8BSU2
Protein Accession #:
NP_075647.3
Nucleotide Accession #:
NM_023158.6
Species Reactivity:
Mouse
Sample Type:
cell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity:
< 1 pg/ml
Kit Range:
15.6 pg/ml-1,000 pg/ml
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)125357556130349618
St. Dev.621.828.97.925.838.9
%CV4.86.105.196.077.396.29
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CXCL16 ELISA Kit (Mouse) (OKBB00317) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A rat monoclonal antibody specific for CXCL16 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (E.coli, N27-P114) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL16 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse CXCL16 captured in well.
Kit Component:
ComponentAmount
Lyophilized recombinant mouse CXCL16 standard10 ng/tube x 2
Anti-mouse CXCL16 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse CXCL16 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CXCL16.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CXCL16.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine.
Datasheets / Downloads:
OKBB00317
Reconstitution and Storage:
Store at 4C for frequent use, at  -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Target Reference:
1. Matloubian M, David A, Engel S, Ryan J, Cyster J (2000). "A transmembrane CXC chemokine is a ligand for
HIV-coreceptor Bonzo". Nat Immunol 1 (4): 298–304.
2. Abel S, Hundhausen C, Mentlein R, Schulte A, Berkhout T, Broadway N, Hartmann D, Sedlacek R, Dietrich S, Muetze B, Schuster B, Kallen K, Saftig P, Rose-John S, Ludwig A (2004). "The transmembrane CXC-chemokine ligand 16 is induced by IFN-gamma and TNF-alpha and shed by the activity of the disintegrin-like metalloproteinase ADAM10". J Immunol 172 (10): 6362–72.
3. Wilbanks A, Zondlo S, Murphy K, Mak S, Soler D, Langdon P, Andrew D, Wu L, Briskin M (2001). "Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines". J Immunol
166 (8): 5145–54.
4. Matloubian, M.; David, A.; Engel, S.; Ryan, J. E.; Cyster, J. G. : A transmembrane CXC chemokine is a ligand for
HIV-coreceptor Bonzo. Nature Immun. 1: 298-304, 2000.
Specificity:
There is no detectable cross-reactivity with other relevant proteins.
Additional Information:
Range: 15.6pg/ml-1000pg/ml
:::
Principle: Aviva’s mouse CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CXCL16 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL16 amount of sample captured in plate.
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using.
Background: Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17p13. The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.
Protocol:
Tips Information:
 
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