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CX3CL1 ELISA Kit (Human) : 96 Wells (OKEH00060)

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96 Wells
$360.00
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Gene Symbol:
CX3CL1
Official Gene Full Name:
C-X3-C motif chemokine ligand 1
NCBI Gene Id:
6376
Alias Symbols:
A-152E5.2, ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, C-X3-C motif chemokine 1, CXC3, CXC3C, FKN, fractalkine, Fractalkine, neurotactin, Neurotactin, NTN, NTT, SCYD1, Small-inducible cytokine D1
Description of Target:
The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
Protein Name:
Fractalkine
Swissprot Id:
P78423
Protein Accession #:
NP_004110
Nucleotide Accession #:
NM_001304392
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.11 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 10.8% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CX3CL1 ELISA Kit (Human) (OKEH00060) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CX3CL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CX3CL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CX3CL1 captured in well.
Kit Component:
ComponentAmount
CX3CL1 Microplate96 Wells (12 x 8 Well strips)
CX3CL1 Lyophilized Standard2
100X Biotinylated CX3CL1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CX3CL1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CX3CL1.
Datasheets / Downloads:
Printable datasheet for OKEH00060
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human Fractalkine. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
42 kDa
Protein Size (# AA):
397
Protocol:
Tips Information:
 
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