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CUBN ELISA Kit (Human) (OKCD02475)

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96 Wells
$545.00
In Stock
Gene Symbol:
CUBN
Official Gene Full Name:
cubilin
NCBI Gene Id:
8029
Alias Symbols:
460 kDa receptor, Cubilin, FLJ90055, FLJ90747, gp280, IFCR, Intestinal intrinsic factor receptor, Intrinsic factor-cobalamin receptor, Intrinsic factor-vitamin B12 receptor, MGA1
Description of Target:
Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
Protein Name:
Cubilin
Swissprot Id:
O60494
Protein Accession #:
NP_001072.2
Nucleotide Accession #:
NM_001081.3
Species Reactivity:
Human
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.112 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level CUBN was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CUBN was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Duration:
3 Hours
Kit Principle:
Aviva Systems Biology CUBN ELISA Kit (Human) (OKCD02475) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CUBN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for CUBN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CUBN captured in well.
Kit Component:
ComponentAmount
Anti-CUBN Microplate96 Wells (12 x 8 Well strips)
CUBN Lyophilized Standard2
100X Biotinylated CUBN Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CUBN.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CUBN.
Datasheets / Downloads:
Printable datasheet for OKCD02475
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Cubilin (CUBN). No significant cross-reactivity or interference between Cubilin (CUBN) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protein Size (# AA):
3623
Protocol:
Tips Information:
 
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