Aviva Systems Biology office will be closed for Thanksgiving - November 23rd, 2017 and November 24th, 2017.
Please go here for more info.

Now Offering Over 102,157 Antibodies & 44,722 Antigens!

CRYGD ELISA Kit (Human) (OKEH06560)

Scroll Horizontally to view all Images
Print Page
  • Let us Help: Ask a Question
96 Wells
$440.00
In Stock
Gene Symbol:
CRYGD
NCBI Gene Id:
1421
Alias Symbols:
CACA, CCA3, CCP, CRYG4, cry-g-D, Gamma-crystallin 4, Gamma-crystallin D, Gamma-D-crystallin
Description of Target:
Crystallins are the dominant structural components of the vertebrate eye lens.
Protein Name:
Gamma-crystallin D
Swissprot Id:
P07320
Protein Accession #:
NP_000246
Nucleotide Accession #:
NM_000255
Species Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
0.156 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 7.9% (n = 20)
Mean Inter-Assay Precision - <= 9.4% (n = 20)
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology CRYGD ELISA Kit (Human) (OKEH06560) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRYGD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRYGD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRYGD captured in the well.
Kit Component:
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Recovery:
Mean recovery when spiking into Serum and Plasma = 83%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CRYGD.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CRYGD.
Datasheets / Downloads:
Printable datasheet for OKEH06560
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protocol:
Tips Information:
 
X
Ask a Question

Tell us what you think about this item!

Write A Review
    Please, wait...