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Clonorchis Sinensis Antibody (IgG) (Human ) ELISA Kit (OKCA00452)

96 Wells
In Stock
Alias Symbols:
Description of Target:
Clonorchis Sinensis Antibody (IgG)
Protein Name:
Clonorchis Sinensis Antibody (IgG)
Species Reactivity:
Sample Type:
Kit Reproducibility:
Three different samples of known Clonorchis Sinensis Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology Clonorchis Sinensis Antibody (IgG) (Human ) ELISA Kit (OKCA00452) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Clonorchis Sinensis antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for Human IgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Clonorchis Sinensis Antibody (IgG) captured in well.
Kit Component:
Clonorchis Sinensis Antibody (IgG) Microplate96 Wells (12 x 8 Well strips)
Negative Control 1 x 150 uL
Positive Control 1 x 150 uL
Anti-Human IgG HRP-conjugate 1 x 10 mL
Sample Diluent 1 x 6 mL
25X Wash Buffer1 x 20 mL
Substrate A 1 x 6 mL
Substrate B 1 x 6 mL
Stop Solution 1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Qualitative Reverse Capture ELISA
Tips Information:

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