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CENPM antibody - middle region (ARP46323_P050)

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100 ul

Regular Price: $289.00

Special Price: $215.00

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Conjugation Options

ARP46323_P050-FITC Conjugated

ARP46323_P050-HRP Conjugated

ARP46323_P050-Biotin Conjugated

Gene Symbol:
CENPM
NCBI Gene Id:
79019
Official Gene Full Name:
Centromere protein M
Protein Name:
Centromere protein M
Swissprot Id:
Q9NSP4
Protein Accession #:
NP_076958
Nucleotide Accession #:
NM_024053
Alias Symbols:
C22orf18, CENP-M, MGC861, PANE1, bK250D10.2
Replacement Item:
This antibody may replace item sc-366262 from Santa Cruz Biotechnology.
Description of Target:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM].
Protein Size (# AA):
180
Molecular Weight:
20kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
IF, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CENPM.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CENPM.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human CENPM
Species Reactivity:
Cow, Dog, Horse, Human, Pig, Rabbit
Predicted Homology Based on Immunogen Sequence:
Cow: 79%; Dog: 79%; Horse: 93%; Human: 100%; Pig: 93%; Rabbit: 93%
Complete computational species homology data:
Anti-CENPM (ARP46323_P050)
Peptide Sequence:
Synthetic peptide located within the following region: CDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
CENPP; CENPL; CENPT; CENPU; CENPO; CENPK; CENPN; CENPQ; CENPI; CENPC; CENPA; UBC;
Blocking Peptide:
For anti-CENPM (ARP46323_P050) antibody is Catalog # AAP46323 (Previous Catalog # AAPS18008)
Datasheets/Manuals:
Printable datasheet for anti-CENPM (ARP46323_P050) antibody
Target Reference:
Izuta,H., (2006) Genes Cells 11 (6), 673-684

Customer Reviews for CENPM Antibody (ARP46323_P050) tested with DNA/ACA in Immunofluorescence

Validation Type:
IF

We tested these antibodies for immunofluorescence using both formaldehyde fixation and methanol fixation. We also tried pre-extraction with detergent for the antibodies.

For the CENP antibodies, these should localize to the centromere. To test this localization, we co-localized these antibodies with ACA (anti-centromere antibodies). The CENP-M antibody (and to a lesser extent CENP-I) did show some punctate foci that co-localized with the ACA.
-Iain Cheeseman

Product Protocols: CENPM antibody tested with Human Hepg2 Cells (ARP46323_P050)

Aviva Systems Biology is the original manufacturer of this CENPM antibody (ARP46323_P050)

Click here to view the CENPM antibody Western Blot Protocol

Product Datasheet Link: CENPM antibody (ARP46323_P050)

WB Suggested Anti-CENPM Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2

Western Blot image:


Description of Target: The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM].

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s CENPM antibody (ARP46323_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
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