website statistics

Now Offering Over 102,157 Antibodies & 44,722 Antigens!

CDK4 antibody - C - terminal region (OAAB17082)

Scroll Horizontally to view all Images
Print Page
  • Let us Help: Ask a Question
400ul
$289.00
In Stock
Gene Symbol:
CDK4
NCBI Gene Id:
1019
Protein Name:
Cyclin-dependent kinase 4
Swissprot Id:
P11802
Protein Accession #:
NP_000066.1
Alias Symbols:
CDK4, Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3
Replacement Item:
This antibody may replace item sc-136241 from Santa Cruz Biotechnology.
Description of Target:
Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight:
33730 Da
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB, IHC, FC, IF
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CDK4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CDK4.
Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets / Downloads:
OAAB17082
Sample Type Confirmation:

CDK4 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Specificity:
This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273~305 amino acids from the C-terminal region of human CDK4.
Isotype:
Ig
Intended Use:
CDK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Application Info:
WB ~~1:1000
IHC ~~1:50 - 100
FC ~~1:10 - 50
IF~~1:10 - 50
:::
Function: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex
:::
Cellular Location: Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus
Additional Product Images:
Protocol:
Tips Information:

Product Protocol: CDK4 antibody tested with human 293, CDK4 by western blot (OAAB17082)

Please find the protocol used to generate the western blot data for item (OAAB17082) by going here

Product datasheet link: CDK4 antibody (OAAB17082)

Western blot analysis of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody (C-term) (Cat. # OAAB17082). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2) (Origene Technologies) .

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CDK4 antibody (OAAB17082) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: CDK4 antibody tested with human 293 by western blot (OAAB17082)

Please find the protocol used to generate the western blot data for item (OAAB17082) by going here

Product datasheet link: CDK4 antibody (OAAB17082)

Western blot analysis of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody (C-term) (Cat. # OAAB17082). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2) (Origene Technologies) .

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CDK4 antibody (OAAB17082) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: CDK4 antibody tested with human hepatocarcinoma in immunohistochemistry (IHC) (OAAB17082)

Please go here to see the protocol used for Aviva's CDK4 antibody (OAAB17082)

Product datasheet can be found here: CDK4 antibody (OAAB17082)

IHC information:
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CDK4 antibody (OAAB17082) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: CDK4 antibody tested with human prostate carcinoma in immunohistochemistry (IHC) (OAAB17082)

Please go here to see the protocol used for Aviva's CDK4 antibody (OAAB17082)

Product datasheet can be found here: CDK4 antibody (OAAB17082)

IHC information:
Formalin-fixed and paraffin-embedded human prostate carcinoma with CDK4 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CDK4 antibody (OAAB17082) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
X
Ask a Question

Tell us what you think about this item!

Write A Review
    Please, wait...