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Cdk16 ELISA Kit (Rat) (OKEH02142)

96 Wells
$490.00
In Stock
Application:
ELISA
Gene Symbol:
CDK16
Official Gene Full Name:
cyclin-dependent kinase 16
NCBI Gene Id:
81741
Alias Symbols:
Cdk16, Cell division protein kinase 16, Cyclin-dependent kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1
Description of Target:
cyclin-dependent kinase related protein that may function in differentiation [RGD, Feb 2006]
Protein Name:
Cyclin-dependent kinase 16
Swissprot Id:
Q63686
Protein Accession #:
NP_112339.1
Nucleotide Accession #:
NM_031077.1
Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 25 pg/mL
Kit Range:
78 - 5,000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Cdk16 ELISA Kit (Rat) (OKEH02142) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk16 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk16 captured in well.
Kit Component:
ComponentAmount
Cdk16 Microplate96 Wells (12 x 8 Well strips)
Cdk16 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cdk16 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CDK16.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CDK16.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat Cyclin-dependent kinase 16. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative sandwich Immunoassay
Molecular Weight:
56 kDa
Protein Size (# AA):
496
Protocol:
Tips Information:

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