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CD9 ELISA Kit (Human) (OKCD00751)

96 Wells
$535.00
In Stock
Application:
ELISA
Gene Symbol:
CD9
Official Gene Full Name:
CD9 molecule
NCBI Gene Id:
928
Alias Symbols:
5H9 antigen, BA2, BTCC-1, CD9 antigen, Cell growth-inhibiting gene 2 protein, DRAP-27, FLJ99568, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, MRP-1, p24, P24, Tetraspanin-29, TSPAN29, Tspan-29, TSPAN-29
Description of Target:
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.3 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.11"Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA."_x005F_x000D_ Ikeyama S., Koyama M., Yamaoko M., Sasada R., Miyake M._x005F_x000D_ J. Exp. Med. 177:1231-1237(1993) [PubMed] [Europe PMC] [Abstract]Cited for: ROLE IN CELL MOTILITY AND METASTASIS.Ref.12"CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin."_x005F_x000D_ Masellis-Smith A., Shaw A.R._x005F_x000D_ J. Immunol. 152:2768-2777(1994) [PubMed] [Europe PMC] [Abstract]Cited for: ROLE IN CELL ADHESION.Ref.13"Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion."_x005F_x000D_ Higginbottom A., Takahashi Y., Bolling L., Coonrod S.A., White J.M., Partridge L.J., Monk P.N._x005F_x000D_ Biochem. Biophys. Res. Commun. 311:208-214(2003) [PubMed] [Europe PMC] [Abstract]Cited for: ROLE IN SPERM-EGG FUSION.
Protein Name:
CD9 antigen
Swissprot Id:
P21926
Protein Accession #:
NP_001760
Nucleotide Accession #:
NM_001769.3
Species Reactivity:
Human
Sample Type:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity:
< 12.5 pg/mL
Kit Range:
31.25-2,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level CD9 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CD9 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology CD9 ELISA Kit (Human) (OKCD00751) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD9captured in well.
Kit Component:
ComponentAmount
Anti-CD9 Microplate96 Wells (12 x 8 Well Strips)
CD9 Lyophilized Standard2 x 2 ng
100X Biotinylated CD9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-95%98-105%90-101%97-104%
EDTA plasma(n=5)78-96%88-95%94-101%86-98%
heparin plasma(n=5)92-104%79-102%78-104%78-105%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)83-9086
EDTA plasma(n=5)96-104101
heparin plasma(n=5)97-105101
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD9.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD9.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Motility Related Protein (MRP1). No significant cross-reactivity or interference between Motility Related Protein (MRP1) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
228
Protocol:
Tips Information:

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