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CD33 ELISA Kit (Human) (OKEH03363)

96 Wells
$495.00
In Stock
Gene Symbol:
CD33
Official Gene Full Name:
CD33 molecule
NCBI Gene Id:
945
Alias Symbols:
FLJ00391, gp67, Myeloid cell surface antigen CD33, p67, Sialic acid-binding Ig-like lectin 3, SIGLEC3, Siglec-3, SIGLEC-3
Description of Target:
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Protein Name:
myeloid cell surface antigen CD33 isoform 2 precursor
Swissprot Id:
P20138
Protein Accession #:
NP_001076087.1
Nucleotide Accession #:
NM_001082618.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 78 pg/mL
Kit Range:
78 - 5,000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 6.5% (n = 20)
Mean Inter-assay CV%: <= 11.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CD33 ELISA Kit (Human) (OKEH03363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD33 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CD33 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD33 captured in well.
Kit Component:
ComponentAmount
Anti-CD33 Microplate96 Wells (12 x 8 Well strips)
CD33 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CD33 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD33.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD33.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural human Myeloid cell surface antigen CD33. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
40 kDa
Protein Size (# AA):
364
Protocol:
Tips Information:

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