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CD248 ELISA Kit (Mouse) (OKCA01662)

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96 Wells
$600.00
In Stock
Gene Symbol:
CD248
Official Gene Full Name:
CD248 antigen, endosialin
NCBI Gene Id:
70445
Alias Symbols:
CD164L1, MGC119478, MGC119479, TEM1, 2610111G01Rik|CD164 sialomucin-like 1|CD248 antigen, endosialin|endosialin|tumor endothelial marker 1
Description of Target:
May play a role in angiogenesis or vascular function.
Protein Name:
Endosialin
Swissprot Id:
Q91V98
Protein Accession #:
NP_473383.1
Nucleotide Accession #:
NM_054042.2
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates
Sensitivity:
1.95 pg/mL
Kit Range:
7.81 - 500 pg/mL
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology Cd248 ELISA Kit (Mouse) (OKCA01662) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd248 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd248 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cd248 captured in the well.
Kit Component:
ComponentAmount
Cd248 Microplate96 Wells (12 x 8 Well strips)
Cd248 Lyophilized Standard2 x 500 pg
100X Biotinylated Cd248 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
1:1009590-99
1:20010296-107
1:4009389-98
1:8008680-92
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9793-102
EDTA Plasma (n=4)9894-103
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Cd248.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Cd248.
Datasheets / Downloads:
Printable datasheet for OKCA01662
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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