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CD160 ELISA Kit (Human) (OKCD01195)

96 Wells
$515.00
In Stock
Application:
ELISA
Gene Symbol:
CD160
Official Gene Full Name:
CD160 molecule
NCBI Gene Id:
11126
Alias Symbols:
BY55, CD160 antigen, FLJ46513, Natural killer cell receptor BY55, NK1, NK28
Description of Target:
Receptor showing broad specificity for both classical and non-classical MHC class I molecules.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.3"MHC class I triggering by a novel cell surface ligand costimulates proliferation of activated human T cells."_x005F_x000D_ Agrawal S., Marquet J., Freeman G.J., Tawab A., Bouteiller P.L., Roth P., Bolton W., Ogg G., Boumsell L., Bensussan A._x005F_x000D_ J. Immunol. 162:1223-1226(1999) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.
Protein Name:
CD160 antigen
Swissprot Id:
O95971
Protein Accession #:
NP_008984
Nucleotide Accession #:
NM_007053.3
Species Reactivity:
Human
Sample Type:
Serum, plasma, cell lysatesand other biological fluids.
Sensitivity:
< 30 pg/mL
Kit Range:
78.12-5,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level CD160 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CD160 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology CD160 ELISA Kit (Human) (OKCD01195) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD160 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD160 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD160captured in well.
Kit Component:
ComponentAmount
Anti-CD160 Microplate96 Wells (12 x 8 Well Strips)
CD160 Lyophilized Standard2 x 5 ng
100X Biotinylated CD160 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)96-104%98-105%86-93%80-95%
EDTA plasma(n=5)78-90%95-102%82-104%84-99%
heparin plasma(n=5)95-104%78-95%85-102%80-92%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)95-103101
EDTA plasma(n=5)98-105101
heparin plasma(n=5)96-10599
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD160.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD160.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 160 (CD160). No significant cross-reactivity or interference between Cluster Of Differentiation 160 (CD160) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
181
Protocol:
Tips Information:

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