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CCNB1 ELISA Kit (Mouse) (OKCA01670)

96 Wells
$610.00
In Stock
Gene Symbol:
CCNB1
Official Gene Full Name:
cyclin B1
NCBI Gene Id:
268697
Alias Symbols:
CCNB, G2/mitotic-specific cyclin B1
Description of Target:
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Protein Name:
G2/mitotic-specific cyclin-B1
Swissprot Id:
P24860
Protein Accession #:
NP_758505.2
Nucleotide Accession #:
NM_172301.3
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
3.9 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology Ccnb1 ELISA Kit (Mouse) (OKCA01670) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccnb1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccnb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Ccnb1 captured in the well.
Kit Component:
ComponentAmount
Ccnb1 Microplate96 Wells (12 x 8 Well strips)
Ccnb1 Lyophilized Standard2 x 1 ng
100X Biotinylated Ccnb1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat8985-94
1:210298-107
1:49187-96
1:88985-93
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)8781-92
EDTA Plasma (n=4)9385-97
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Ccnb1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Ccnb1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:

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