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CCNB1 Antibody (OAAF00681)

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100 ug
$289.00
In Stock

Conjugation Options

OAAF00681-FITC Conjugated

OAAF00681-HRP Conjugated

OAAF00681-Biotin Conjugated

Gene Symbol:
CCNB1
NCBI Gene Id:
891
Official Gene Full Name:
G2/mitotic-specific cyclin-B1
Protein Name:
G2/mitotic-specific cyclin-B1
Swissprot Id:
P14635
Protein Accession #:
NP_114172.1
Alias Symbols:
CCNB,
Replacement Item:
This antibody may replace item sc-166757 from Santa Cruz Biotechnology.
Host:
Rabbit
Clonality:
Polyclonal
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Application:
WB, IHC, IF, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCNB1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCNB1.
Immunogen:
The antiserum was produced against synthesized peptide derived between 91-140 amino acids from the Internal region of human Cyclin B1.
Species Reactivity:
Human, Mouse
Product Format:
Liquid
Reconstitution and Storage:
Store at -20C/1 year
Datasheets / Downloads:
OAAF00681
Specificity:
Cyclin B1 (Ab-126) antibody detects endogenous levels of total Cyclin B1 protein.
Formulation:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution:
The optimal working dilution should be determined by the end user.
Application Info:
WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
Target Reference:
Jing Yang, J. Biol. Chem., Jan 2001; 276: 3604 - 3609.
Susan Walsh, Mol. Cancer Res., Feb 2003; 1: 280.
Florian Bassermann, J. Biol. Chem., Mar 2007; 10.1074.
Jianke Li, PNAS, Jan 1997; 94: 502.
Protocol:
Tips Information:

Product Protocol: CCNB1 antibody tested with HeLa in western blot (WB) (OAAF00681)

Please go here to see the protocol used for Aviva's CCNB1 antibody (OAAF00681)

Product datasheet can be found here: CCNB1 antibody (OAAF00681)

WB information:
Western blot analysis of extracts from HeLa cells, treated with serum (10%, 15min), using Cyclin B1 (Ab-126) antibody (#BOO68, Line 1 and 2). The lane on the right is treated with the synthesized peptide.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CCNB1 antibody (OAAF00681) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: CCNB1 antibody tested with human breast carcinoma in immunohistochemistry (IHC) (OAAF00681)

Please go here to see the protocol used for Aviva's CCNB1 antibody (OAAF00681)

Product datasheet can be found here: CCNB1 antibody (OAAF00681)

IHC information:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Ab-126) antibody (#BOO68). The picture on the right is treated with the synthesized peptide.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CCNB1 antibody (OAAF00681) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Protocol: CCNB1 antibody tested with HeLa in immunofluorescence (IF) (OAAF00681)

Please go here to see the protocol used for Aviva's CCNB1 antibody (OAAF00681)

Product datasheet can be found here: CCNB1 antibody (OAAF00681)

IF information:
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using Cyclin B1 (Ab-126) antibody (#BOO68). The picture on the right is treated with the synthesized peptide.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology's CCNB1 antibody (OAAF00681) has been tested using other cell lysates and tissues. To obtain more data about this antibody, please email us at info@avivasysbio.com

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva's products are guaranteed for the applications and experimental sample types mentioned. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

 
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