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CCL5 ELISA Kit (Canine) : 96 Wells (OKCA00238)

96 Wells
$480.00
In Stock
Gene Symbol:
CCL5
Official Gene Full Name:
chemokine (C-C motif) ligand 5
NCBI Gene Id:
403522
Alias Symbols:
C-C motif chemokine 5, SCYA5, Small-inducible cytokine A5, T-cell-specific protein RANTES
Description of Target:
This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
Protein Name:
C-C motif chemokine 5
Swissprot Id:
Q8HYS0
Protein Accession #:
NP_001003010
Nucleotide Accession #:
NM_001003010
Species Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue homogenates
Sensitivity:
< 0.081 ng/mL
Kit Range:
0.156 ng/mL - 10 ng/mL
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (ng/ml)1.2341.2081.2531.2251.2271.202
SD0.0260.0220.0280.0460.0490.051
CV%2.3772.0352.5394.2244.4954.731
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology CCL5 ELISA Kit (Canine) (OKCA00238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Canine CCL5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Canine CCL5 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Canine CCL5 captured in well.
Kit Component:
ComponentAmount
Anti-CCL5 Microplate96 Well (12 x 8 Well Strips)
CCL5 Standard (Lyophilized)2 x 10 ng
100X CCL5 Detection Antibody1 x 120 uL
100X HRP-Avidin1 x 120 uL
Detection Antibody Diluent1 x 15 mL
HRP-Avidin Diluent1 x 15 mL
Sample Diluent1 x 50 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Linearity:
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:1105%97-110%
Serum (n=4)1:2101%95-106%
Serum (n=4)1:496%87-101%
Serum (n=4)1:8102%97-107%
Kit Recovery:
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)104%96-108%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)99%91-104%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCL5.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCL5.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of dog CCL5. No significant cross-reactivity or interference between dog CCL5 and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
10 kDa
Protein Size (# AA):
91
Protocol:
Tips Information:

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